Plant tissue extraction for metabolomics.
نویسندگان
چکیده
Plants are not only important producers of foods and energy storages (e.g., sugars, carbohydrates, proteins, and fats) in the form of grains, fruits, and vegetables, they also provide many valuable products to human existence including wood, fibers, oils, resins, pigments, antioxidants, and sources of medicine. Most importantly in light of this book, plants have been a source of therapeutic and health promoting compounds throughout history. This chapter describes several essential considerations for the extraction process when aiming to study plant metabolism or to characterize the chemical composition of plant originated samples using metabolomics technologies.
منابع مشابه
An optimized method for NMR-based plant seed metabolomic analysis with maximized polar metabolite extraction efficiency, signal-to-noise ratio, and chemical shift consistency.
Plant metabolomic analysis has become an essential part of functional genomics and systems biology and requires effective extraction of both primary and secondary metabolites from plant cells. To establish an optimized extraction method for the NMR-based analysis, we used the seeds of mungbean (Vigna radiata cv. Elü no. 1) as a model and systematically investigated the dependence of the metabol...
متن کاملFrom Models to Crop Species: Caveats and Solutions for Translational Metabolomics
Although plant metabolomics is largely carried out on Arabidopsis it is essentially genome-independent, and thus potentially applicable to a wide range of species. However, transfer between species, or even between different tissues of the same species, is not facile. This is because the reliability of protocols for harvesting, handling and analysis depends on the biological features and chemic...
متن کاملA Simple Fractionated Extraction Method for the Comprehensive Analysis of Metabolites, Lipids, and Proteins from a Single Sample
Understanding of complex biological systems requires the measurement, analysis and integration of multiple compound classes of the living cell, usually determined by transcriptomic, proteomic, metabolomics and lipidomic measurements. In this protocol, we introduce a simple method for the reproducible extraction of metabolites, lipids and proteins from biological tissues using a single aliquot p...
متن کاملGas Chromatography Mass Spectrometry Coupling Techniques
Relative to other metabolomics analysis techniques, gas chromatography mass spectrometry (GC/MS) is one of the earliest applied analysis techniques in metabolomics. The first paper on metabolomics (metabolic profiling) is derived from the application of GC/MS analysis in urine and tissue extracts (Dalgliesh et al. 1966). With the arrival of omics era and the proposing of metabolomics concept, p...
متن کاملPlant metabolomics: the missing link in functional genomics strategies.
After the establishment of technologies for high-throughput DNA sequencing (genomics), gene expression analysis (transcriptomics), and protein analysis (proteomics), the remaining functional genomics challenge is that of metabolomics . Metabolomics is the term coined for essentially comprehensive, nonbiased, high-throughput analyses of complex metabolite mixtures typical of plant extracts. This...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
عنوان ژورنال:
- Methods in molecular biology
دوره 1055 شماره
صفحات -
تاریخ انتشار 2013